DAYA HAMBAT REBUSAN DAUN ALPUKAT (Persea americana Mill.) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli

Abstract

Avocado plants including medicinal plants, part of the avocado plants that can cure diseases are usually leaves, stems, roots and fruit are used as a remedy in the form of stew. Avocado leaf (Persea americana Mill.) Has several active substances such as flavonoids, saponins, steroids and tannins that are anti-bacterial substances. In this research, it is done to test the inhibition of avocado leaf to the growth of Escherichia coli bacteria in order to know the concentration of avocado leaf stew which is able to inhibit the growth of Escherichia coli bacteria.

            This research is a laboratory experiment using dilution test method. The sample used in this research is avocado leaf (Persea americana Mill.) Which is boiled, then the stew is diluted to 100%, 80%, 60%, 40% and 20% concentration. As a control concentration of 0% contains only sterile aquadest without the addition of avocado leaves decoction on EMB (Eosin Methylen Blue) media.

From the research results obtained the decoction of avocado leaves at concentrations of 20% - 100% can inhibit the growth of Escherichia coli bacteria. The higher the concentration of avocado leaves stew given the lower the growth rate of Escherichia coli bacteria, because of the substance contained avocado leaf. It can be concluded that there is influence of avocado leaf sensitivity to growth of Escherichia coli bacteria.